Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 76, Issue 10, Pages 3378-3382Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02715-09
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Funding
- NWO-ERGO
- NATO Science [ESP.EAP.CLG 981785]
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Four extraction methods, including a novel one, were compared for their efficiencies in producing DNA from three contrasting agricultural soils. Molecular analyses (PCR-denaturing gradient gel electrophoresis [DGGE] and clone libraries) focusing on different microbial groups were used as assessment criteria. Per soil, the DNA yields differed between extraction methods. Clear effects of method on apparent richness and community structure were found. Actinobacterial diversity based on soil DNA produced by two divergent methods revealed that a hitherto-undescribed group was obtained by the novel method.
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