Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 76, Issue 22, Pages 7658-7661Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01083-10
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- Inje University
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Here, we report cloning of cyanobacterial genes encoding pteridine glycosyltransferases that catalyze glucosyl or xylosyl transfer from UDP-sugars to tetrahydrobiopterin. The genes were cloned by PCR amplification from genomic DNA which was isolated from culture and environmental samples and overexpressed in Escherichia coli for an in vitro activity assay.
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