Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 76, Issue 14, Pages 4896-4900Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02846-09
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Funding
- Norwegian Research Council [180444/V40]
- NSERC
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All cultivated Thermotogales are thermophiles or hyperthermophiles. However, optimized 16S rRNA primers successfully amplified Thermotogales sequences from temperate hydrocarbon-impacted sites, mesothermic oil reservoirs, and enrichment cultures incubated at <46 degrees C. We conclude that distinct Thermotogales lineages commonly inhabit low-temperature environments but may be underreported, likely due to universal 16S rRNA gene primer bias.
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