Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 75, Issue 23, Pages 7537-7541Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01541-09
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Funding
- College of Natural Resources
- Environment at the University of Massachusetts
- Sloan Foundation
- National Science Foundation [0743432]
- Austrian GEN-AU project BIN
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0743432] Funding Source: National Science Foundation
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mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the alpha and beta diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.
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