Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 75, Issue 9, Pages 2925-2930Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02470-08
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Funding
- Portuguese Institute Fundacao para a Ciencia e Tecnologia [SFRH/BD/29297/2006, SFRH/BPD/42208/2007]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/42208/2007, SFRH/BD/29297/2006] Funding Source: FCT
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Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.
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