Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 75, Issue 10, Pages 3352-3354Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02407-08
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Funding
- Macquarie University Research Fellowship
- Macquarie University Research and Development Grant
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Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.
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