Journal
STEM CELLS
Volume 23, Issue 7, Pages 895-902Publisher
WILEY
DOI: 10.1634/stemcells.2004-0326
Keywords
human embryonic stem cells; pluripotency; differentiation; feeder-free
Categories
Funding
- MRC [G0301182] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/B/14779] Funding Source: researchfish
- Medical Research Council [G0301182] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BBS/B/14779] Funding Source: Medline
- Medical Research Council [G0301182] Funding Source: Medline
Ask authors/readers for more resources
One of the most frequently used matrices for feeder-free growth of undifferentiated human embryonic stem cells (hESCs) is Matrigel, which supports attachment and growth of undifferentiated hESCs in the presence of mouse embryonic fibroblast-conditioned medium. Unfortunately, application of Matrigel or medium conditioned by mouse embryonic feeder cells is not ideal for potential medical application of hESCs because xenogeneic pathogens can be transmitted through culture conditions. We demonstrate here that human serum as matrix and medium conditioned by differentiated hESCs reduce exposure of hESCs to animal ingredients and provide a safer direction toward completely animal-free conditions for application, handling, and understanding of hESC biology. At the same time, hESCs grown under these conditions maintain all hESC features after prolonged culture, including the developmental potential to differentiate into representative tissues of all three embryonic germ layers, unlimited and undifferentiated proliferative ability, and maintenance of normal karyotype.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available