Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 75, Issue 23, Pages 7556-7559Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01014-09
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Funding
- National Institutes of Health
- National Library of Medicine
- Israel Science Foundation [434/02]
- Israeli Ministry of Science and Technology
- EMBO YIP award
- U.S. National Science Foundation [NSF-0950550, OCE-0550547]
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To detect anoxygenic bacteria containing either type 1 or type 2 photosynthetic reaction centers in a single PCR, we designed a degenerate primer set based on the bchY gene. The new primers were validated in silico using the GenBank nucleotide database as well as by PCR on pure strains and environmental DNA.
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