4.6 Article

Genetic Analysis for the Lack of Expression of the O157 Antigen in an O Rough:H7 Escherichia coli Strain

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 76, Issue 3, Pages 945-947

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02046-09

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Funding

  1. Center for Food Safety and Applied Nutrition

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The O-antigen (rfb) operon and related genes of MA6, an O rough:H7 Shiga-toxigenic Escherichia coli strain, were examined to determine the cause of the lack of O157 expression. A 1,310-bp insertion, homologous to IS629, was observed within its gne gene. trans complementation with a functional gne gene from O157: H7 restored O157 antigen expression in MA6.

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