Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 76, Issue 4, Pages 1298-1300Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02190-09
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Funding
- National Natural Science Foundation of China [20606017]
- 973 Program of China [2009CB724701]
- Qing Lan Project of Jiangsu Province
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Aerobic growth conditions significantly influenced anaerobic succinate production in two-stage fermentation by Escherichia coli AFP111 with knockouts in rpoS, pflAB, ldhA, and ptsG genes. At a low cell growth rate limited by glucose, enzymes involved in the reductive arm of the tricarboxylic acid cycle and the glyoxylate shunt showed elevated activities, providing AFP111 with intracellular redox balance and increased succinic acid yield and productivity.
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