4.6 Article

Cellulase Production from Spent Lignocellulose Hydrolysates by Recombinant Aspergillus niger

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 75, Issue 8, Pages 2366-2374

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02479-08

Keywords

-

Funding

  1. Carl Fredrik von Horn's Foundation
  2. South African-Swedish Research Collaboration Programme of Sida/SAREC
  3. NRF

Ask authors/readers for more resources

A recombinant Aspergillus niger strain expressing the Hypocrea jecorina endoglucanase Cel7B was grown on spent hydrolysates ( stillage) from sugarcane bagasse and spruce wood. The spent hydrolysates served as excellent growth media for the Cel7B-producing strain, A. niger D15[egI], which displayed higher endoglucanase activities in the spent hydrolysates than in standard medium with a comparable monosaccharide content ( e. g., 2,100 nkat/ml in spent bagasse hydrolysate compared to 480 nkat/ml in standard glucose-based medium). In addition, A. niger D15[egI] was also able to consume or convert other lignocellulose-derived compounds, such as acetic acid, furan aldehydes, and phenolic compounds, which are recognized as inhibitors of yeast during ethanolic fermentation. The results indicate that enzymes can be produced from the stillage stream as a high-value coproduct in secondgeneration bioethanol plants in a way that also facilitates recirculation of process water.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.6
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available