Journal
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
Volume 56, Issue 2, Pages 413-415Publisher
OXFORD UNIV PRESS
DOI: 10.1093/jac/dki228
Keywords
bacteriocins; UTIs; uropathogens
Funding
- NICHD NIH HHS [K23 HD042014-01, K23 HD042014-03, K23 HD042014-02, HD42014, K23 HD042014] Funding Source: Medline
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Objectives: Natural microbial defence systems, such as bacteriocins, may be a novel means to prevent catheter-associated urinary tract infection. We investigated in vitro whether a colicin-expressing strain of Escherichia, coli could prevent urinary catheter colonization by a colicin-susceptible, uropathgenic strain of E. coli. Methods: Segments of urinary catheter were inoculated with colicin-producing E. coli K-12 and then exposed to either colicin-susceptible E. coli (a uropathogenic clinical isolate) or colicin-resistant E. coli (derived from the susceptible clinical isolate). Catheters were then incubated overnight, rinsed and sonicated. Results: The presence of colicin-producing E. coli K-12 on the catheter surface completely prevented catheter colonization by colicin-susceptible E. coli but not by resistant E. coli. The colicin-susceptible strain but not the colicin-resistant strain also disappeared from broth cultures in the presence of colicin-producing E. coli K-12. Conclusions: The observed inhibition of catheter colonization by the uropathogenic clinical isolate of E. coli can be attributed to the presence of a colicin-producing strain of E. coli on the catheter surface. Bacteriocin production by a non-pathogenic organism may have clinical applicability as a means to prevent catheter-associated urinary tract infection.
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