Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 75, Issue 18, Pages 6017-6021Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00211-09
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- University of North Carolina at Charlotte
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We present a simple strategy for isolating and accurately enumerating target DNA from high-clay-content soils: desorption with buffers, an optional magnetic capture hybridization step, and quantitation via real-time PCR. With the developed technique, mu g quantities of DNA were extracted from mg samples of pure kaolinite and a field clay soil.
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