4.6 Article

Effect of Freezing on PCR Amplification of 16S rRNA Genes from Microbes Associated with Black Band Disease of Corals

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 75, Issue 8, Pages 2581-2584

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01500-08

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Funding

  1. NIH [NIH/NIGMS SO6GM8205]

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Molecular analysis of black band disease of corals revealed that samples frozen immediately after collection yielded more proteobacterial 16S rRNA sequences, while unfrozen samples produced more cyanobacterial and sulfur-oxidizing bacterial sequences. These results suggest the need to use multiple approaches for preparation of samples to characterize this complex polymicrobial disease.

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