Journal
FUNGAL GENETICS AND BIOLOGY
Volume 42, Issue 8, Pages 726-735Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2005.04.007
Keywords
Blumeria graminis; Erysiphe graminis; demethylase inhibiting fungicides; fungicide resistance; CYP51 gene; cytochrome P450 sterol 14 alpha-demethylase; triadimenol
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Resistance to sterol 14 alpha-demethylase inhibiting fungicides (DMIs) has been correlated with mutations in the CYP51 gene, which encodes the target enzyme eburicol 14 alpha-demethylase. To test the hypothesis that variation in the CYP51 gene explains variation for DMI sensitivity in barley and wheat powdery mildew species, this gene was sequenced from isolates of Blumeria graminis f.sp. hordei (Bgh) and f.sp. tritici (Bgt), respectively, which differed in their responses to DMIs in agricultural Populations in the UK. Two single nucleotide mutations in the CYP51 gene, which resulted in the arnino acid substitutions Y136F and K147Q, were detected. K147Q is a novel mutation present only in Bgh isolates expressing very high levels of resistance. Sequence analysis of the CYP51 gene from the progeny or a cross between DMI-sensitive and resistant Bgh isolates showed that both mutations segregate with resistance, which is consistent with CYP51 controlling a major portion of DMI resistance. However, genetic analysis of resistance to the DMI triadimenol indicates that mutation of the CYP51 gene is not the only mechanism of resistance operating in B. graminis. (c) 2005 Elsevier Inc. All rights reserved.
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