Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 74, Issue 5, Pages 1660-1663Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02403-07
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We investigated the effects of internal primer-template mismatches on the efficiency of PCR amplification using the 16S rRNA gene as the model template DNA. We observed that the presence of a single mismatch in the second half of the primer extension sequence can result in an underestimation of up to 1,000-fold of the gene copy number, depending on the primer and position of the mismatch.
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