Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 75, Issue 1, Pages 252-256Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01630-08
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Funding
- Australian Research Council
- Hermon Slade Foundation
- Centre for Marine Bio-Innovation at the University of New South Wales, Australia
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A novel method has been developed for the selective extraction of DNA from surface-associated bacterial communities from the two model marine benthic algae Ulva australis and Delisea pulchra. The extracted DNA had no detectable contamination with host DNA, was recovered in high yield and quality, and was representative of the bacterial community on the algal surfaces. The DNA is suitable for a variety of subsequent applications, including the construction of large-insert clone libraries and metagenomic sequencing.
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