Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 74, Issue 22, Pages 7051-7055Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01511-08
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To avoid potentiating the spread of an antibiotic resistance marker, a plasmid expressing a leuB gene and a heterologous antigen, green fluorescent protein (GFP), was shown to complement a leucine auxotroph of cattle vaccine strain Brucella abortus RB51, which protected CD1 mice from virulent B. abortus 2308 and elicited GFP antibodies.
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