Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 74, Issue 10, Pages 3302-3305Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02630-07
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Funding
- NIGMS NIH HHS [P01 GM077596] Funding Source: Medline
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We describe here an improved method for isolating, purifying and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide solution. This method was used to construct large-insert soil metagenomic libraries.
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