4.6 Article

PBAD-Based Shuttle Vectors for Functional Analysis of Toxic and Highly Regulated Genes in Pseudomonas and Burkholderia spp. and Other Bacteria

Journal

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 74, Issue 23, Pages 7422-7426

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01369-08

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Funding

  1. National Aeronautics and Space Administration (NASA) [NNA04CC74G, NNX06AH20H]
  2. NIH [AI065357]

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We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the PBAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.

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