Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 74, Issue 23, Pages 7422-7426Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01369-08
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Funding
- National Aeronautics and Space Administration (NASA) [NNA04CC74G, NNX06AH20H]
- NIH [AI065357]
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We report the construction of a series of Escherichia-Pseudomonas broad-host-range expression vectors utilizing the PBAD promoter and the araC regulator for routine cloning, conditional expression, and analysis of tightly controlled and/or toxic genes in pseudomonads.
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