Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 74, Issue 12, Pages 3912-3914Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00127-08
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We developed a two-step isothermal amplification assay system, which achieved the detection of norovirus (NoV) genomes in oysters with a sensitivity similar to that of reverse transcription-seminested PCR. The time taken for the amplification of NoV genomes from RNA extracts was shortened to about 3 h.
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