Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 75, Issue 2, Pages 551-556Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01527-08
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Funding
- National Natural Science Foundation of China [20506037, 20672037]
- Ministry of Science and Technology, People's Republic of China [2006AA02Z205, 2007AA02Z225]
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A new and efficient sulfide monooxygenase-producing strain, ECU0066, was isolated and identified as a Rhodococcus sp. that could transform phenylmethyl sulfide (PMS) to (S)-sulfoxide with 99% enantiomeric excess via two steps of enantioselective oxidations. Its enzyme activity could be effectively induced by adding PMS or phenylmethyl sulfoxide (PMSO) directly to a rich medium at the early log phase (6 h) of fermentation, resulting in over 10-times-higher production of the enzyme. This bacterial strain also displayed fairly good activity and enantioselectivity toward seven other sulfides, indicating a good potential for practical application in asymmetric synthesis of chiral sulfoxides.
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