Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 74, Issue 24, Pages 7824-7827Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.01712-08
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- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
- University of Buenos Aires (UBA) in Argentina
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We describe a new enzymatic functionality for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356, namely, an endopeptidase activity against the cell wall of Salmonella enterica serovar Newport, assayed via zymograms and identified by Western blotting. Based on amino acid sequence comparisons, the hydrolase activity was predicted to be located at the C terminus. Subsequent cloning and expression of the C-terminal domain in Bacillus subtilis resulted in the functional verification of the enzymatic activity.
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