Journal
MACROMOLECULAR BIOSCIENCE
Volume 5, Issue 8, Pages 717-727Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/mabi.200500061
Keywords
alginate; enzyme immobilization; layer-by-layer self assembly; microspheres; nanofilms
Funding
- NIBIB NIH HHS [R01 EB000739-02, R01 EB000739, R01 EB000739-03] Funding Source: Medline
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In an effort to improve the stability for long-term biosensor use, layer-by-layer self-assembly was explored as a potential technique to provide a diffusion barrier to encapsulated glucose oxidase inside alginate microspheres (<5 mu m), fabricated using an emulsification technique. The total loss of encapsulated enzyme was reduced to less than 25 and 15% with the application of single PAH/PSS and crosslinked PAH/PAA coatings, respectively, in comparison to at least a 45% loss observed with uncoated and PDDA/PSS-coated microspheres. Furthermore, it was found that enzyme within PAH/PSS- and crosslinked PAH/PAA-coated spheres retained more than 84 and 60% of initial activity, respectively, after three months, whereas uncoated and PDDA/ PSS-coated microspheres retained less than 20%.
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