Journal
BIOSENSORS & BIOELECTRONICS
Volume 21, Issue 2, Pages 378-383Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2004.07.037
Keywords
surface plasmon resonance; mixed self-assembled monolayers; glutamic acid decarboxylase; streptavidin; immunosensor
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To enhance the feasibility of surface plasmon resonance (SPR) immunosensor as a tool for diagnosing type I diabetes, we enhanced the sensitivity of immunoresponse for detecting the monoclonal anti-glutamic acid decarboxylase (GAD) antibody by modification of mixed self-assembled monolayers (SAMs). The effects of the different mixed SAMs were evaluated with respect to the degree of streptavidin immobilization, the degree of biotin-GAD immobilization, and the immunoresponse sensitivity. Consequently, the sensitivity of the immunoresponse for the detection of anti-GAD antibody was enhanced as a result of the reduction in steric hindrance brought about by using SAMs of heterogeneous lengths. The immunoresponse for detecting the monoclonal anti-GAD antibody was also enhanced with the reduction of the excess immobilization of biotin-GAD and the minimization of non-specific binding that resulted from the simple substitution of the spacer from a carboxylic-terminated SAM for the hydroxyl-terminated SAM. (c) 2004 Elsevier B.V. All rights reserved.
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