Journal
FEMS MICROBIOLOGY LETTERS
Volume 249, Issue 2, Pages 233-240Publisher
OXFORD UNIV PRESS
DOI: 10.1016/j.femsle.2005.06.015
Keywords
Azoarcus; BAC library; physical map; genome sequencing; functional genomics
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Azoarcus sp. strain BH72 is a Gram-negative proteobacterium of the beta subclass; it is a diazotrophic endophyte of graminaceous plants and can provide significant amounts of fixed nitrogen to its host plant Kallar grass. We aimed to obtain a physical map of the Azoarcus sp. strain BH72 chromosome to be directly used in functional analysis and as a part of an Azoarcus sp. BH72 genome project. A bacterial artificial chromosome (BAC) library was constructed and analysed. A representative physical map with a high density of marker genes was developed in which 64 aligned BAC clones covered almost the entire genome. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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