Journal
FEBS LETTERS
Volume 579, Issue 20, Pages 4226-4234Publisher
WILEY
DOI: 10.1016/j.febslet.2005.06.053
Keywords
golgi; monoclonal antibody; RNA interference; syntaxin; vesicular transport
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It has been suggested that syntaxin 5 (Syx5) participates in vesicular transport. We examined the effects of Syx5 down-regulation on the morphology of the Golgi apparatus and the transport of vesicles in mammalian cells. Knockdown of the Syx5 gene resulted in Golgi fragmentation without changing the level of endoplasmic reticulum (ER)-resident proteins, other Golgi-SNARES (soluble N-ethylmaleimide-sensitive factor-attachment protein receptors), and coatmer proteins. Strikingly, a major decrease in Syx5 expression barely affected the anterograde transport of vesicular stomatitis virus G (VSVG) protein to the plasma membrane. These results suggest that Syx5 is required for the maintenance of the Golgi structures but may not play a major role in the transport of vesicles carrying VSVG between the ER and the Golgi compartment. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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