Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 102, Issue 33, Pages 11606-11610Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0502078102
Keywords
electrochemistry; hole transport; ferrocene; gold electrode
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Establishing a reliable genotyping protocol is a critical matter in postsequence genetics. In this article, we describe a highly sensitive electrochemical detection of complementary DNAs (up to 43-mer) based on hole transport with molecular-scale, wire-like DNA probes. The presence of a single-base mismatch in the DNA duplexes caused a dramatic decrease in the electrochemical response. We applied this method to detect all of the possible transition and transversion SNPs and achieved on-off-type discrimination of fully complementary DNAs from their SNPs. Furthermore, naturally occurring polymorphisms, hot spots from the p53 gene, could clearly be distinguished from wild type by using our methodology.
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