Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 334, Issue 2, Pages 433-440Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2005.06.111
Keywords
tRNA methylation; wybutosine; modification of tRNA bases
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Funding
- NIA NIH HHS [AG18000] Funding Source: Medline
- NIGMS NIH HHS [GM26020] Funding Source: Medline
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We demonstrate that the product of the yeast open reading frame YML005w is required for wybutosine (yW) formation in the phenylalanine-accepting tRNA of the yeast Saccharomyces cerevisiae. tRNA isolated from a deletion mutant of the YML0051v gene accumulates 4-demethylwyosine (ImG-14), a precursor lacking three of the methyl groups of the yW hypermodified base. Since the amino acid sequence of the YML005w gene contains the signature motifs of the seven beta-strand methyltransferases, we now designate the gene TRM12 for tRNA methyltransferase. Using pulse-chase labeling of intact yeast cells with S-adenosyl-L-[methyl/H-3]methionine, we show that the methylesterified form of yW is metabolically stable. (c) 2005 Elsevier Inc. All rights reserved.
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