Journal
FEBS LETTERS
Volume 579, Issue 21, Pages 4659-4665Publisher
WILEY
DOI: 10.1016/j.febslet.2005.07.036
Keywords
NIMA-related kinase; cell cycle progression; meristems; higher plants
Ask authors/readers for more resources
Meristems are sites of undifferentiated cell division, which carry on developing into functional organs. Using the two-hybrid system with a poplar 14-3-3, we uncovered poplar NIMA-related kinase 1 (PNek1) as an interacting protein. PNek1 shows high homology to the mammalian NIMA-related kinases, which are thought to be involved in cell cycle progression. Using a synchronized poplar cell suspension, we observed an accumulation of PNek1 mRNA at the GIN transition and throughout the G(2)-to-M progression. Moreover, PNek1-GFP fusion protein localized in the cytoplasm and in both the nuclear and nucleolar regions. Overexpression of PNek1-GFP in Arabidopsis caused morphological abnormalities in flower and siliques. Overall, these results suggest that PNek1 is involved in plant development. Crown copyright (c) 2005 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available