Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 102, Issue 35, Pages 12612-12617Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0504172102
Keywords
genetically engineered crops; low phytate; signal transduction; phosphate sensing
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Funding
- NHLBI NIH HHS [R01 HL-55672, R01 HL055672] Funding Source: Medline
- NIDDK NIH HHS [R33 DK-070272, R33 DK070272] Funding Source: Medline
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Phytate (inositol hexalkisphosphate, IN is a regulator of intracellular signaling, a highly abundant animal antinutrient, and a phosphate store in plant seeds. Here, we report a requirement for inositol polyphosphate kinases, AtIPK1 and AtIPK2 beta, for the later steps of phytate synthesis in Arabidopsis thaliana. Coincident disruption of these kinases nearly ablates seed phytate without accumulation of phytate precursors, increases seed-free phosphate by 10-fold, and has normal seed yield. Additionally, we find a requirement for inositol tetrakisphosphate (IP4)/inositol pentakisphosphate ON 2-kinase activity in phosphate sensing and root hair elongation. Our results define a commercially viable strategy for the genetic engineering of phytate-free grain and provide insights into the role of inositol polyphosphate kinases in phosphate signaling biology.
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