Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 128, Issue 1-2, Pages 47-53Publisher
ELSEVIER
DOI: 10.1016/j.jviromet.2005.04.001
Keywords
genotypic assay; RNase H region; non-B subtypes; gag cleavage sites
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Genotypic assays are used often to guide clinicians in decisions concerning the treatment of patients. An optimized sequence-based genotypic assay was used to determine the whole protease and reverse transcriptase (RT) gene, including the gag cleavage site region and RNase H region. Since non-B subtypes are increasing in countries where subtype B was the most prevalent Subtype, and treatment becomes more available in developing countries where the epidemic is characterized by a high prevalence of non-B subtypes, it was important that the genotypic test was evaluated using a panel of different subtypes. Amplification was successful for different subtypes: A, B, C, D, F, G, H, J, CRF01_AE, CRF02-AG, CRF11-cpx, CRF13-cpx and an uncharacterized recombinant sample. The detection limit of the PCR was 1000 copies/ml, except for 1 subtype C sample (PL3) and 1 CRF02_AG sample (PL8). The detection limit for these samples was 5000 copies/ml. A sequence could be obtained in both directions for most of the samples. (c) 2005 Elsevier B.V. All rights reserved.
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