Journal
BIOCHEMISTRY-MOSCOW
Volume 70, Issue 9, Pages 1020-1026Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1007/s10541-005-0219-8
Keywords
muscle glycogen phosphorylase b; guanidine hydrochloride; inactivation; aggregation; osmolyte
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The effects of the osmolytes trimethylamine-N-oxide (TMAO), betaine, proline, and glycine on the kinetics of inactivation and aggregation of rabbit skeletal muscle glycogen phosphorylase b by guanidine hydrochloride (GuHCl) have been studied. It is shown that the osmolytes TMAO and betaine exhibit the highest protective efficacy against phosphorylase b inactivation. A test system for studying the effects of macromolecular crowding induced by osmolytes on aggregation of proteins is proposed. TMAO and glycine increase the rate of phosphorylase b aggregation induced by GuHCl.
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