Journal
NANO LETTERS
Volume 5, Issue 9, Pages 1819-1823Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nl0507360
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Funding
- NIGMS NIH HHS [R01 GM065364-04, GM067445, GM065364, R01 GM065364, F32 GM067445] Funding Source: Medline
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This paper describes a technique for growing filamentous cells of Escherichia coli with defined shapes, including crescents, zigzags, sinusoids, and spirals. The procedure begins with the fabrication of embossed microchambers in agarose. Cells are trapped in the chambers by placing flat, flexible ceiling, either a slab of agarose or poly(dimethylsiloxane), against an agarose mold on which a suspension of cells has been added; the use of agarose keeps cells hydrated and allows nutrients to diffuse into the chambers. Cells grown in microchambers in the presence of cephalexin grow into a multinucleate, nonseptate, filamentous phenotype and adopt the shape of the microchambers. The resulting cells are motile and can be released by removing the ceiling from the agarose microchambers and rinsing the cells into solution.
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