Transcription factor GATA-3 is involved in repression of promoter activity of the human interleukin-4 gene

GATA-3 was shown to bind to two sites of the IL-4 gene promoter in human T-cell lines PER-I 17 and Jurkat. A motif located in the region of position -860 and responsible for GATA-3 binding was detected for the first time. Mutation or deletion of this site increased the promoter activity. The findings suggest a direct involvement of GATA-3 in regulation of the human IL-4 gene transcription as a repressor of the promoter activity.