Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 39, Issue 1-2, Pages 187-195Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jpba.2005.02.039
Keywords
tears; mucins; contact lenses; sialic acids; electrophoresis
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Characteristics of tear-film may be influenced by contact lens wear, because contact lenses present the habitual, direct rubbing action of the lids upon the covered ocular surface and may cause changes of tear-film. In the present paper, influence of contact lens on proteins in tear samples was studied using carbohydrates attached to the protein as a marker. We found that N-acetylneuraminic acid (Neu5Ac) was significantly decreased in tear samples of volunteers wearing contact lens (wearing, 86.1 +/- 57.7 nmol/ml; normal, 190.2 +/- 121.9 nmol/ml). Analysis by polyacrylamide gel electrophoresis demonstrated that the amounts of major proteins in tear fluids, such as lactoferrin and secretory immunoglobulin A were not changed upon wearing contact lenses. In contrast, cellulose acetate membrane electrophoresis revealed that mucin band in tear samples from contact lens wearers showed significant decrease as examined by lectin staining. (c) 2005 Elsevier B.V. All rights reserved.
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