Endotoxin exposure alters brain and liver effects of fumonisin B1 in BALB/c mice:: Implication of blood brain barrier

Fumonisin B-1 (FB1), a mycotoxin produced by Fusarium verticillioides, causes equine leukoencephalomalacia and hepatotoxicity. We studied the modulation of FBI toxicity in brain and liver of female BALB/c mice after endotoxin administration to compromise the blood-brain barrier (BBB) integrity. Mice were injected intraperitoneally with saline or 3 mg/kg of lipopolysaccharide (LPS) followed 2 It later by either a single or three daily subcutaneous doses of 2.25 mg/kg of FBI. After 4 It of a single FBI injection the inhibition of sphingolipid biosynthesis occurred in liver. Circulating alanine aminotransferase increased by LPS alone at this time. In brain LPS triggered inflammation increasing the expression of tumor necrosis factor (TNF) alpha, interferon (IFN) gamma, interleukin (IL)-1 beta, IL-6, and IL-12; no effect of FB1 was observed. In liver LPS + FB1 attenuated the expression TNF alpha and IFN gamma compared to LPS alone. One day after the 3-day FB1 treatment the biosynthesis of sphingolipids was markedly reduced in brain and liver and it was further inhibited when LPS was given before FBI. FBI induced hepatotoxicity, as measured by circulating liver enzymes, was reduced after the combined treatment with LPS + FB1 compared to FB1 alone. FB1 decreased the LPS-induced brain expression of IFN gamma and IL-1 beta, whereas the expression of IL-6 and IL-12 was augmented. In liver FB1 also reduced the expression of IL-1 beta and IFN gamma compared to LPS alone. Results indicated that endotoxemia concurrent with FBI intoxication facilitated the permeability of fumonisin in brain indicated by increased accumulation of sphinganine and endotoxin modified the effects of FBI in both brain and liver. (c) 2005 Elsevier Ltd. All rights reserved.