Journal
NATURE CELL BIOLOGY
Volume 7, Issue 9, Pages 901-U57Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/ncb1293
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Funding
- NIGMS NIH HHS [R01 GM047214, R01 GM47214] Funding Source: Medline
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Growth of normal cells is anchorage dependent because signalling through multiple pathways including Erk, phosphatidylinositol- 3- OH kinase ( PI( 3) K) and Rac requires integrin- mediated cell adhesion(1). Components of these pathways localize to low- density, cholesterol- rich domains in the plasma membrane named ' lipid rafts'(2,3) or 'cholesterol-enriched membrane microdomains' ( CEMM)(4). We previously reported that integrin- mediated adhesion regulates CEMM transport such that cell detachment from the extracellular matrix triggers CEMM internalization and clearance from the plasma membrane(5). We now report that this internalization is mediated by dynamin- 2 and caveolin- 1. Internalization requires phosphorylation of caveolin- 1 on Tyr 14. A shift in localization of phospho- caveolin- 1 from focal adhesions to caveolae induces CEMM internalization upon cell detachment, which mediates inhibition of Erk, PI( 3) K and Rac. These data define a novel molecular mechanism for growth and tumour suppression by caveolin- 1.
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