Journal
APMIS
Volume 122, Issue 6, Pages 545-551Publisher
WILEY
DOI: 10.1111/apm.12183
Keywords
Circulating tumor cells; CTC; cancer; CytoTrack
Categories
Funding
- Toyota-Fonden, Denmark
- Familien Erichsens Mindefond
- Fabrikant Ejner Willumsens Mindelegat
- Hilleroed Hospital Forskningspulje
- Beckett-Fonden
- Olga Bryde Nielsens Fond
- Harboefonden
- Tvergaards fond
- Andersen-Isted Fonden
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Analysis of circulating tumor cells (CTC) holds promise of providing liquid biopsies from patients with cancer. However, current methods include enrichment procedures. We present a method (CytoTrack((R))), where CTC from 7.5mL of blood is stained, analyzed and counted by a scanning fluorescence microscope. The method was validated by breast cancer cells (MCF-7) spiked in blood from healthy donors. The number of cells spiked in each blood sample was exactly determined by cell sorter and performed in three series of three samples spiked with 10, 33 or 100 cells in addition with three control samples for each series. The recovery rate of 10, 33 and 100 tumor cells in a blood sample was 55%, 70% and 78%, percent coefficient of variation (CV%) for samples was 59%, 32% and 18%, respectively. None of the control samples contained CTC. In conclusion, the method has been validated to highly sensitively detect breast cancer cells in spiking experiments and should be tested on blood samples from breast cancer patients. The method could benefit from automation that could reduce the CV%, and further optimization of the procedure to increase the recovery.
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