Nocardioides dubius sp nov., isolated from an alkaline soil

A Gram-positive, rod- or coccus-shaped bacterial strain, KSL-104(T), was isolated from an alkaline soil from Korea and its taxonomic position was investigated by a polyphasic approach, Strain KSL-104(T) grew optimally at pH 7(.)0-8(.)0 and 30 degrees C. It was characterized chemotaxonomically as having a cell-wall peptidoglycan type based on (LL)-2,6-diaminopimelic acid with MK-8(H-4) as the predominant menaquinone. The major fatty acid was iSO-C-16:0 and the major polar lipids; were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The DNA G + C content was 70(.)6 mol%. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain KSL-104(T) joined the cluster comprising Nocardioides jensenii and Marmoricola aurantiacus. The cellular fatty acid profile of strain KSL-104(T) was different from that of M. aurantiacus. Strain KSL-104(T) and the type strain of N. jensenii exhibited a 16S rRNA gene sequence similarity value of 97(.)1 % and a mean DNA-DNA relatedness value of 13 %. Levels of 16S rRNA gene sequence similarity between strain KSL-104 T and the type strains of other Nocardioides species were in the range 94(.)0-96(.)5%. On the basis of phenotypic, phylogenetic and genetic data, strain KSL-104(T) (= KCTC 9992(T)=JCM 13008(T)) represents a novel species of the genus Nocardioides, for which the name Nocardioides dubius sp. nov. is proposed.