Journal
APMIS
Volume 117, Issue 12, Pages 900-904Publisher
WILEY
DOI: 10.1111/j.1600-0463.2009.02552.x
Keywords
Neisseria gonorrhoeae; prolyliminopeptidase; NG-MAST; pip and porB gene sequencing; Sweden
Categories
Funding
- Orebro County Council Research Committee
- Foundation for Medical Research at Orebro University Hospital, Sweden
Ask authors/readers for more resources
In Neisseria gonorrhoeae culture diagnostics, species confirmation is commonly performed using commercial biochemical tests relying on prolyliminopeptidase (PIP) activity. It was previously shown that one PIP-negative strain was mainly globally transmitted during 2000-2004. The aims were to investigate the prevalence and phenotypic and genetic characteristics of PIP-negative N. gonorrhoeae isolates in Sweden during 2000-2007. Gonococcal isolates (n = 1230) cultured in Sweden during 2000-2007 were characterized using PIP screening, antibiogram, serovar determination, pip and porB gene sequencing, and N. gonorrhoeae multiantigen sequence typing (NG-MAST). Fifteen (1.2%) of the isolates were PIP-negative. Of those, 13 (87%) were indistinguishable to the previously described globally transmitted strain, i.e. displayed serovar IB-4 (Bpyvut), similar antibiograms, ST210 (n = 10)/ST292 (n = 3) and contained an identical single nucleotide pip gene deletion. Wherever high reliance is placed on PIP activity for N. gonorrhoeae species confirmation, changes in the diagnostic strategies may need to be considered and/or monitoring of the PIP activity is crucial.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available