Journal
BRAIN RESEARCH PROTOCOLS
Volume 15, Issue 3, Pages 115-118Publisher
ELSEVIER
DOI: 10.1016/j.brainresprot.2005.05.002
Keywords
cell cycle; synchronization; serum starvation; confluence; G0
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Funding
- NCI NIH HHS [5 P30 CA46592] Funding Source: Medline
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Serum starvation of astrocytes for a period of time followed by refeeding has been proposed as a method to produce synchronized astrocytes. Here, it is proposed that the method neither synchronizes cells nor satisfies rigorous criteria for cell synchronization. The proposed non-selective, whole-culture synchronization protocol cannot, in theory, synchronize cells. The cells produced by the proposed serum starvation/refeeding protocol do not reflect the properties of any particular cell during the cell cycle. Cells produced by the published protocol will have a wide distribution of cell sizes, and therefore, the cells produced by starvation/refeeding will not model cells of any specific age during the division cycle. Thus, the proposed protocol will not produce a synchronized culture. (c) 2005 Elsevier B.V All rights reserved.
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