Journal
MAGNETIC RESONANCE IN MEDICINE
Volume 54, Issue 3, Pages 718-724Publisher
JOHN WILEY & SONS INC
DOI: 10.1002/mrm.20617
Keywords
magnetic resonance imaging; cardiomyocyte; apoptosis; reperfusion; nanoparticle
Funding
- NCI NIH HHS [R24 CA92782, CA91807, R01CA86782, P50CA86355] Funding Source: Medline
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The ability to image cardiomyocyte apoptosis in vivo with high-resolution MRI could facilitate the development of novel cardioprotective therapies. The sensitivity of the novel nanoparticle AnxCLIO-Cy5.5 for cardiomyocyte apoptosis was thus compared in vitro to that of annexin V-FITC and showed a high degree of colocalization. MRI was then performed, following transient coronary artery (LAD) occlusion, in five mice given AnxCLIO-Cy5.5 and in four mice given an identical dose (2 mg Fe/kg) of CLIO-Cy5.5. MR signal intensity and myocardial T-2* were evaluated, in vivo, in hypokinetic regions of myocardium in the LAD distribution. Ex vivo fluorescence imaging was performed to confirm the in vivo findings. Myocardial T2* was significantly lower in the mice given AnxCLIO-Cy5.5 (8.1 versus 13.2 ms, P < 0.01), and fluorescence target to background ratio was significantly higher (2.1 versus 1.1, P < 0.01). This study thus demonstrates the feasibility of obtaining high-resolution MR images of cardiomyocyte apoptosis in Vivo with the novel nanoparticle, AnxCLIO-Cy5.5.
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