Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 53, Issue 9, Pages 1167-1170Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1369/jhc.5B6691.2005
Keywords
immunohistochemistry; antigen retrieval; standardization protein-embedding-technique beads
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Funding
- NCI NIH HHS [1 R33 CA-103455-01] Funding Source: Medline
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A serial study was performed to develop a protein-embedding technique for standardization of immunohistochemistry (IHC) on formalin-fixed, paraffin-embedded (FFPE) tissue sections. A protein carrier matrix must have two phases, a liquid phase to allow uniform mixing of a protein and a solid phase forming a 'block' that can be fixed and processed in the same manner as human tissue. This standardized protein block would serve as a source of thin sections for control of IHC and therefore must also withstand the boiling conditions of antigen retrieval (AR). After multiple experiments, a method was developed utilizing polymer microsphere (beads) as a support medium for protein. The method showed particular promise for quantitative IHC.
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