Journal
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
Volume 1, Issue 3, Pages 352-358Publisher
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jbn.2005.041
Keywords
In Vitro Cytotoxicity; Gold Nanoparticles; Violacein; Antitumor; Drug Delivery; HL60
Funding
- Brazilian Nanobiotechnology Network (CNPq/MCT)
- Millennium Institute for Complex Materials (CNPq)
- FAPESP
Ask authors/readers for more resources
In vitro cytotoxicity of a supramolecular system composed by violacein complexed by,beta-cyclodextrin-thiol-protected gold nanoparticles (violacein @ beta-CD-S(CH2)(6)-S-Au) on V79 and HL60 cell lines is described. The gold nanoparticles were prepared and modified in a single step involving reduction of tetrachloroaurate ions with sodium borohydride in the presence of thiol derivatized beta-cyclodextrin. Inclusion complexation of violacein into cyclodextrin cavities occurred by mixing an aqueous solution of the gold nanoparticles with an acetone solution of violacein, being evidenced by UV/visible absorption spectroscopy According to cell viability measurements based on the MTT assay (3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide) the supramolecular system was found to maintain the cytotoxic effects compared to free violacein on HL60 cells and in addition is less cytotoxic to normal (V79) cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available