Journal
EXPERIMENTAL EYE RESEARCH
Volume 81, Issue 3, Pages 326-339Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exer.2005.02.005
Keywords
integrin; cell adhesion; lens development; lens differentiation; fibroblast growth factor
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Funding
- NEI NIH HHS [EY03177] Funding Source: Medline
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Mammalian lens development involves cell-cell and cell-ECM interactions. As integrins are a major family of cell adhesion molecules, we examined the expression patterns of several integrin subunits (alpha 3A, alpha 3B, alpha 6A, alpha 6B, beta 1 and beta 4) during rat lens development. RT-PCR, in situ hybridisation, immunofluorescence and immunoblotting were used to investigate expression of integrin subunits during lens development and differentiation. RT-PCR showed expression of alpha 3A, alpha 6A, alpha 6B and beta 1A but not alpha 3B or beta 4 subunits in postnatal rat lenses. Each subunit displayed distinct spatio-temporal expression patterns. beta 1 integrin was expressed in both epithelium and fibres. alpha 3A subunit expression was restricted to the epithelium; expression ceased abruptly at the lens equator. Expression of the alpha 6A subunit increased during fibre differentiation, whereas alpha 6B expression was predominantly associated with epithelial cells during lens development. In lens epithelial explants, FGF induced some of the changes in integrin expression that are characteristic of fibre differentiation in vivo. One notable exception was the inability of FGF to reproduce the distinctive down-regulation of the alpha 3 isoform that is associated with initiation of elongation in vivo. Interestingly, vitreous treatment was able to reproduce this shift in alpha 3 expression indicating that another factor(s), in addition to FGF, may be required for full and complete transition from an epithelial cell to a fibre cell. Integrin subunit expression therefore appears to be highly regulated during lens development and fibre differentiation with evidence of major changes in alpha 3 and alpha 6 isoform expression. These results indicate that integrins may play important roles in development and growth of the lens. How specific integrin subunits influence the behaviour of cells in different developmental compartments of the lens remains to be determined. (c) 2005 Elsevier Ltd. All rights reserved.
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