Journal
MOLECULAR AND BIOCHEMICAL PARASITOLOGY
Volume 143, Issue 1, Pages 39-48Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molbiopara.2005.04.012
Keywords
ASP; parasitic transition; hookworm; mixed model ANOVA; microarray
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Funding
- NIAID NIH HHS [R01 AI042908] Funding Source: Medline
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Ancylostoma caninum is a common canine parasite responsible for anemia and death in infected dogs. Gene expression profiling was used to investigate molecular differences between two different forms of the third larval stage (Us): infective free-living larvae and in vitro serum-stimulated larvae that mimic the initial stages of parasitism of a host. We developed an A. caninum cDNA microarray consisting of 4191 EST clones, and used it to identify a set of It 3 genes that are differentially regulated between infective and parasitic larval stages. Real-time RT-PCR was used to confirm the expression differences of a subset of the genes. Of the genes repressed upon serum stimulation, seven encode members of the `Ancylostoma secreted protein' ASP family, while another transcript encoding a 24 kDa excretory protein with similarity to ASP was up-regulated in serum-stimulated L3s. This suggests that different members of a protein family that has important implications for the hookworm's parasitic lifestyle are regulated in a complementary manner in response to serum stimulation. Comparison of two strains of A. caninum from North Carolina and Maryland only identified a single gene, one of the members of the ASP family, that was differentially repressed upon serum stimulation. (c) 2005 Elsevier B.V. All rights reserved.
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