Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 62, Issue 3, Pages 337-344Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2005.04.017
Keywords
nuclear magnetic resonance spectroscopy; microscopy; imaging; Shewanella oneidensis strain MR-1; biofilm; metabolite analysis; L-[3-C-13]lactate
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Novel procedures and instrumentation are described for nuclear magnetic resonance (NMR) spectroscopy and imaging studies of live, in situ microbial films. A perfused NMR/optical microscope sample chamber containing a planar biofilm support was integrated into a recirculation/dilution flow loop growth reactor system and used to grow in situ Shewanella oneidensis strain MR-1 biofilms. Localized NMR techniques were developed and used to non-invasively monitor time-resolved metabolite concentrations and to image the biomass volume and distribution. As a first illustration of the feasibility of the methodology an initial C-13-labeled lactate metabolic pathway study was performed, yielding results consistent with existing genomic data for MR-1. These results represent progress toward our ultimate goal of correlating time- and depth-resolved metabolism and mass transport with gene expression in live in situ biofilms using combined NMR/optical microscopy techniques. (c) 2005 Elsevier B.V. All rights reserved.
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