Journal
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
Volume 124, Issue 3, Pages 404-407Publisher
OXFORD UNIV PRESS INC
DOI: 10.1309/6EA3U9V8NCLLGKQN
Keywords
real-time PCR; blood cultures; Staphylococcus aureus
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We developed, validated, and implemented real-time polymerase chain reaction (PCR) detection of the femA gene for Staphylococcus aureus and the mecA gene for methicillin resistance directly from BACTEC (Becton Dickinson, Sparks, MD) blood culture bottles showing gram-positive cocci in clusters. For the 332 positive blood cultures tested, the assay had 100% sensitivity and specificity for identifying methicillin-susceptible (n = 28) and methicillin-resistant (n = 28) S aureus, and overall was 98% sensitive and 94% specific, with 3 uninterpretable test results when identification of coagulase-negative staphylococci was included. PCR detection yields rapid (2-3 hours) results and accurate identification of S aureus directly from signal-positive blood culture bottle samples.
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