Journal
PHYTOCHEMISTRY
Volume 66, Issue 17, Pages 2032-2037Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phytochem.2004.12.003
Keywords
polyphenols; cysteine; antioxidants; glutamate; neuroprotection
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Funding
- NINDS NIH HHS [NS28121] Funding Source: Medline
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Antioxidant compounds derived from the conjugation of (-)-epicatechin and (-)-epicatechin 3-Omicron-gallate with cysteine and cysteine derivatives protected HT-22 nerve cells (EC50 between 36 and 65 mu M) from death triggered by glutamate while underivatized (-)-epicatechin was almost inactive (EC50 = 610 mu M). Differences in free radical scavenging capacity (DPPH assay) could not account for the improvement in neuroprotective activity upon derivatization of (-)-epicatechin with thiols. Moreover, while the gallate-containing compounds are more efficient radical scavengers than their non-galloylated counterparts, they are only equally or less potent as neuroprotective agents. Although all of the conjugates were able to scavenge mitochondrially generated reactive oxygen species (ROS) inside the cells, the majority of their neuroprotective activity appeared to be dependent upon their ability to maintain glutathione levels. These results suggest that a, mechanism other than ROS scavenging is involved in the neuroprotective action exerted by the epicatechin conjugates. (c) 2004 Elsevier Ltd. All rights reserved.
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